Sequence of cDNA for a novel light-induced glycine-rich protein.

Irradiation with blue light induces chloroplast differentation in suspension cultured cells of Chenopodium rubrum (1). During this developmental process specific organelle proteins are synthesized de-novo. Concomitantly, a drastic rise in activity level and steady-state concentration of numerous mRNAs is observed (2). A cDNA library was prepared using poly(A)-rich RNA from cells exposed to blue light for 6-24h, and screened for clones which correspond to rapidly light-induced genes. Representative for these is clone HC1. Analysis of both strands of the 689 bp insert by dideoxy chain termination technique with T7 polymerase (3) revealed the complete coding sequence for a glycinrich protein of 144 amino acids which exhibits a domain structure: Residues 9 3 6 form a hydrophobic a-helix whereas the following sequence (37 113) consists of six repeats with two motifs each 6 amino acids long: -Gly-Tyr-Asn-Asn-Gly-Glyand -Gly-Tyr-His-Asn-Gly-Gly-.